Cover of: Biology of the chemokine RANTES |

Biology of the chemokine RANTES

  • 123 Pages
  • 2.66 MB
  • English

Springer-Verlag, R.G. Landes , New York, Austin
Chemokines., T cells., Lymphocyte transformation., Lymphokines -- immunology., T-Lymphocytes -- immuno
Statement[edited by] Alan M. Krensky.
SeriesMolecular biology intelligence unit, Molecular biology intelligence unit (Unnumbered)
ContributionsKrensky, Alan M.
LC ClassificationsQR185.8.C45 B56 1995
The Physical Object
Pagination123 p. :
ID Numbers
Open LibraryOL1275699M
ISBN 101570592535, 3540592202
LC Control Number95007283

Volume 2 of this two-volume set discusses the pathophysiology of chemokines. It is divided into two parts: a) chemokines in animal disease models, and b) chemokines as drug targets.

Together with volume 1, which discusses the immunobiology of chemokines, both volumes give a comprehensive overview of chemokine biology. RANTES is a member of the C-C family of chemoattractant cytokines (chemokines). It is secreted by T lymphocytes late after activation, and by fibroblasts, epithelial cells and endothelial cells after stimulation with tumour necrosis factor-α, interleukin-1β and interferon-γ.

RANTES is a potent chemoattractant for monocytes, memory T cells, basophils and by: The proceedings of that meeting were published in Advances in Experimental Medicine and Biology, vol.

Download Biology of the chemokine RANTES FB2

The rapid advances made in the field of chemotactic cytokines over the last 18 months necessitated a third Symposium in this series to collate and place in. Hypertension was associated with an increase in perivascular adipose tissue expression of the chemokine RANTES (relative quantification, ± vs.

± ; Pchemokine Cited by: Summary Interactions of the chemokine CCL5 (RANTES) with glycosaminoglycans (GAGs) are crucial to the CCL5-mediated inflammation process. However, structural information on interactions between CCL5 and longer GAG fragments is by: Both the CC chemokine ligand 5 (CCL5/RANTES) and interleukin-6 (IL-6), released by mesenchymal stem cells (MSCs) as well as by neoplastic cells, promote breast cancer cell progression through autocrine and paracrine mechanisms.

In order to assess the. The aim of this study was to investigate the role of human herpesvirus-6 (HHV-6) in autoimmune thyroiditis (AIT) development.

We examined the possible involvement of HHV-6 gene expression encoding immunomodulating proteins U12 and U51 in AIT development and their role in the modulation of chemokine signaling. One hundred patients with autoimmune thyroiditis following thyroidectomy. RANTES (Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted) is a chemokine secreted by platelets that have been activated predominantly during flow conditions– This chemokine interacts with P-selectin in mediating monocyte/macrophage infiltration into atherosclerotic lesions.

Abstract RANTES (CCL5) is a chemokine expressed by many hematopoietic and non-hematopoietic cell types that plays an important role in homing and migration of effector and memory T cells during acute infections.

The RANTES receptor, CCR5, is a major target of anti-HIV drugs based on blocking viral entry. Joseph Lorenzo MD, in Osteoimmunology (Second Edition), CCR1. Ligands for CCR1 include CCL3 (MIP-1α), CCL5 (RANTES), CCL7 (MCP-3), CCL13 (MCP-4), CCL14 (HCC-1), CCL15 (LKN-1), and CCL23 (MPIF-1).

Inhibition of CCR1 expression with siRNA, or by blocking NFATc1 activation with cyclosporin A, inhibited migration of RAW cells (a model for osteoclast precursors) and.

The discovery of this fourth type of chemokine raises questions about the evolutionary history of CX 3 C and the other chemokines. The CXC, CC, C and CX 3 C chemokine genes map to human chromosomes 4, 17, 1 respectively.

There are some exceptions, for instance SDF-1 maps to chromos and this chemokine may in fact represent a. Chemokines (Greek -kinos, movement) are a family of small cytokines, or signaling proteins secreted by cells.

Their name is derived from their ability to induce directed chemotaxis in nearby responsive cells; they are chemo tactic cyto kines. Cytokine proteins are classified as chemokines according to behavior and structural ro: IPR Subsequent to the structures of CXCL8 and other CXC chemokines, the structure of the CC chemokine, CCL4/MIP‐1β, was solved, 8 followed by CCL5/RANTES 9 and CCL2/MCP‐1 10 shortly thereafter.

These CC chemokine structures revealed a distinctly different dimer motif compared with CXC dimers. Binding of RANTES/CCL5 to GAGs and to GPCRs are crucial for its pro-inflammatory activity [42–44].

Mutating the main GAG-binding domain in RANTES has been shown to switch the [A 44 ANA 47]-RANTES/CCL5 chemokine to a potent anti-inflammatory molecule in murine models of inflammatory diseases.

The anti-inflammatory properties of a RANTES/CCL5. Migration rate in response to RANTES/CCL5 treatment was measured by modified Boyden chamber experiments. As shown in Fig. 2A, in the absence of stimulation by the chemokine, SDC4WT-GFP, SDC4LQQ-GFP, SDC4Adel-GFP-transfected endothelial cell migration was unchanged as compared to vector-transfected ones (control).By contrast, SDC4SA-GFP-transfected cell.

This chapter describes the methodological approaches to studying the role of chemokines and chemokine receptors in the physiology of immune and inflammatory responses. Chemokines share the common function of attracting leukocytes to sites of an inflammatory or immune response. The G protein-coupled cell surface receptors (GPCRs) as signal transducers of chemotaxis appear to be highly.

shown in Figure Chemokine receptors have been identified that bind chemo-kines but do not signal. One of these, the Duffy antigen receptor for chemokines (DARC) is a promiscuous chemokine receptor expressed on erythrocytes that binds both CC and CXC ligands [12].

Details Biology of the chemokine RANTES FB2

In contrast, the decoy receptor D6 only binds CC chemokines [13]. followed by chemical optimization, we identified a novel non-peptide CCR1 antagonist, R-N-[5-chloro[2-[4-[(4-fluorophenyl)methyl]methylpiperazinyl]oxoethoxy]phenyl]urea hydrochloric acid salt (BX ).

Competition binding studies revealed that BX was able to displace the CCR1 ligands macrophage inflammatory protein-1α. This set of books* gives a state-of-the-art account of recent developments in this field in the form of summaries written by highly regarded experts. Volume I is focused on basic principles and progress in chemokine biology, and Volume II deals with issues related to chemokine-related drug development and potential therapeutic applications.

sion of chemokine biology is beyond the scope of this review. Interested readers are referred to recent review articles devoted to chemokines and their receptors [22–28].

Description Biology of the chemokine RANTES FB2

Inconsistency and bewildering complexity have character-ized the nomenclature of chemokines and their receptors in the past.

It is not uncommon to encounter from three to. Chemokines play an important role in inflammation. The mechanism via which they bind to more than one receptor and activate them is not well understood.

The chemokines are thought to interact with their receptors via two distinct sites, one necessary for binding and the other for activation of signal transduction. This volume in the Current Topics in Membranes series discusses the biology of chemokines and their binding partners, chemokine receptors, in normal and disease-related states.

Chemokines are small proteins that are important in normal immune responses. Recent research demonstrates a role for these proteins in a variety of diseases such as heart disease, allergy, asthma, and cancer.

The CC chemokines MCP-4 and RANTES competed for MCP-3 binding with IC 50 values of and nM, respectively. The chromosomal location of CCR10 was determined to coincide with the CC chemokine receptor cluster on chromosome 3 (3pp).

Eight chemokines were tested for ability to elicit transendothelial chemotaxis of unstimulated peripheral blood T lymphocytes. The C‐C chemokines monocyte chemotactic protein (MCP)‐2, MCP‐3, RANTES. Platelets Secrete an Eosinophil-chemotactic Cytokine which is a Member of the CC Chemokine Family.- Neutrophil-activating Peptide ENA The Effects of Human Recombinant MIP-1?, MIP-1.

and RANTES on the Chemotaxis and Adhesion of T Cell Subsets.- Promiscuity of Ligand Binding in the Human Chemokine Beta Receptor Family Monoclonal antibodies (MAbs) produced through the technique of somatic cell fusion are fundamental tools in the exploration of chemokine biology.

This chapter details the procedures that were used to generate a panel of MAbs directed against the human chemokine RANTES (1, 2). The general approach described here should be broadly applicable to.

Selective attraction of monocytes and T lymphocytes of the memory phenotype by cytokine RANTES. Nature. ; () (Biology) Schall TJ, Jongstra J, Dyer BJ, et al.

A human T cell-specific molecule is a member of a new gene family. J Immunol. ; (3) (Biology) Vaddi K, Keller M, Newton RC. The Chemokines Facts Book. Chemokine (C-C motif) ligand 5 (also CCL5) is a protein which in humans is encoded by the CCL5 gene.

It is also known as RANTES (regulated on activation, normal T cell expressed and secreted). The chemokine RANTES induced biphasic mobilization of Ca2+ in T cells. The initial peak, a transient increase in cytosolic Ca2+ mediated by a heterotrimeric guanine nucleotide-binding protein (G protein)--coupled pathway, was associated predominantly with chemotaxis.

The second peak, Ca2+ release and sustained influx dependent on protein tyrosine kinases, was associated with a spectrum of. Biology of the Chemokine in Rantes (Molecular Biology Intelligence Unit).

R G Landes Co. ISBN ↑ Song A, Chen YF, Thamatrakoln K, Storm TA, Krensky AM (January ). "RFLAT a new zinc finger transcription factor that activates RANTES gene expression in T lymphocytes".

Chemokine structures: similar tertiary structures, but many different oligomeric states. Much is known about the tertiary structure of chemokines from NMR and crystallography [].Paradoxically, despite their diversity of functions and sequences, most chemokines have similar tertiary folds as represented by the monomer structure of MCP-1/CCL2 in Figure 2A.

Interestingly, i.c.v. injection of RANTES increased immobility time in FST (P chemokine exposure can directly result in depressive behavior. Furthermore, we tested the effects of Met‐RANTES, a CCL5 receptor (CCR5) .Inflammatory chemokines and cytokines in obesity and the metabolic syndrome.

Obesity leads to chronic low-grade inflammation of WAT. Activated macrophages (MacroΦ) and T cells within WAT produce increased levels of inflammatory chemokines such as CCL2/MCP-1 and CCL5/RANTES as well as cytokines such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and IL